Structure-Activity Study of the Lantibiotic Mutacin II from Streptococcus mutans T8 by a Gene Replacement Strategy
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منابع مشابه
Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy.
Mutacin II, elaborated by group II Streptococcus mutans, is a ribosomally synthesized and posttranslationally modified polypeptide antibiotic containing unusual thioether and didehydro amino acids. To ascertain the role of specific amino acid residues in mutacin II antimicrobial activity, we developed a streptococcal expression system that facilitates the replacement of the mutA gene with a sin...
متن کاملMutacin II, a Bactericidal Lantibiotic from Streptococcus mutans
MICHAEL L. CHIKINDAS,† JAN NOVÁK, ARNOLD J. M. DRIESSEN,* WIL N. KONINGS, KURT M. SCHILLING,‡ AND PAGE W. CAUFIELD Unilever Dental Research, Bebington L63 3JW, United Kingdom; Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294; and Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of G...
متن کاملThe specific genes for lantibiotic mutacin II biosynthesis in Streptococcus mutans T8 are clustered and can be transferred en bloc.
Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a ...
متن کاملMutacin II, a bactericidal antibiotic from Streptococcus mutans.
Mutacin II is an antibiotic that is produced by group II Streptococcus mutans. It inhibits the growth of other streptococci as well as many other gram-positive microorganisms by a hitherto unknown mechanism. Mutacin II possess bactericidal activity against susceptible cells. It transiently depolarizes the transmembrane electrical potential (delta psi) and the transmembrane pH gradient (delta pH...
متن کاملGenetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans.
Streptococcus mutans JH1000 and its derivatives were previously shown (J. D. Hillman, K. P. Johnson, and B. I. Yaphe, Infect. Immun. 44:141-144, 1984) to produce a low-molecular-weight, broad-spectrum bacteriocin-like inhibitory substance (BLIS). The thermosensitive vector pTV1-OK harboring Tn917 was used to isolate a BLIS-deficient mutant, DM25, and the mutated gene was recovered by shotgun cl...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.7.2335-2340.1998